Table 1.

Summary of diarrhea and fecal VirHRV shedding after VirHRV challenge (PCD 1 to PCD 6).

Groupa	N	Diarrheab			Virus sheddingc
		% of pigs	Mean cumulative fecal scored	Mean duration (days)e	% of pigs	Geometric mean of peak titer (FFU/ml)f	Mean Duration (days)
Deficient HIFM	6	33.3	5.3	1.2	100	797.4**	2.5
Sufficient HIFM	6	0.0	3.8	NA	33.3	35.4*	1.5

^aDeficient HIFM; gnotobiotic (Gn) pigs transplanted with human infant fecal microbiota (HIFM) at 4 days of age (PTD 0) and fed protein deficient diet, Sufficient HIFM; Gn pigs transplanted with HIFM at 4 days of age and fed protein sufficient diet, All pigs were orally vaccinated twice with attenuated human rotavirus Wa strain (AttHRV) on post-transplantation day (PTD) 7 and 17, and challenged with virulent human rotavirus (VirHRV) on PTD 24.

^bPigs with fecal score > 1 were considered as diarrheic. Fecal consistency was scored as follows: 0, normal; 1, pasty; 2, semi-liquid; and 3, liquid.

^cDetermined by cell culture immunofluorescence assay and expressed as FFU/ml.

^dMean of total of fecal scores from PCD 1 to PCD 6.

^eMean of the total days with fecal score > 1.

^fSamples negative for HRV detection (<25) were assigned a titer of 12.5 for statistical analysis. Means in the same column with different asterisks differ significantly