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. 2018 Oct 11;18:967. doi: 10.1186/s12885-018-4738-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — a Immunohistochemical analysis showing expression of VEGF in different treatment groups. b, c Serum levels of VEGF and HIF-1α determined by ELISA assay in different treatment groups. d Representative images of immunohistochemical analysis showing expression of HIF-1α in different treatment groups. e Expression of VEGF and HIF-1α was determined by Western blot analysis. GAPDH served as the loading control. Data are presented as the mean ± SD. ^*P < 0.05, ^**P < 0.01 versus the control group; ^#P < 0.05, ^##P < 0.01 versus the MET NVB+ Endo group