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. 2018 Oct 10;11:278. doi: 10.1186/s13068-018-1270-1

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Enzyme locations in the host strain producing the β-carotene substrate. a Soluble cytoplasmic enzymes (PhCCD1) in the cells. b PhCCD1 localized to the membrane compartment by fusion with GlpF. c CrtW and CrtZ both localized to the membrane separately. d GlpF fused to the fusion protein CrtW-CrtZ to target it to the membrane