Table 1.
Strains and plasmids in this study
| Strains/plasmids | Relevant characteristics | Source/notes |
|---|---|---|
| Strains | ||
| CAR010 | CAR005 [27], mRSL-4::ispG, mRSL-14::ispH | Unpublished |
| CAR003 | CAR010, ∆crtX | Unpublished |
| CAR025 | CAR005 [27], ispG-mRSL-4, ispH-mRSL-14, replacing the promoter of crtEYIB with trc promoter | [22] |
| Plasmids | ||
| pSC101 | Low copy plasmid | Unpublished |
| pACYC184-M | cat; replace tet with lacI and Ptrc of pTrc99A-M | [27] |
| pACYC184M2-Pm46 | cat; kan; expression vector; replace lacI and Ptrc of pAYCA184-M with FRT-Km-FRT::M1-46 | Unpublished |
| pSC102 | Low copy plasmid, ori and repA from pSC101, M1-46 promoter, cat from pACYC184-M2-Pm46 | Unpublished |
| pYL002 | crtW in pSC102 | Unpublished |
| pYL501 | crtW and crtZ in pSC102 | Unpublished |
| pGlpF-CrtW | glpF fused with crtW in pSC102 | This study |
| pCrtZ | crtZ in pSC102 | This study |
| pGlpF-CrtZ | glpF fused with crtZ in pSC102 | This study |
| pGlpF-CrtW/GlpF-CrtZ | glpF fused with crtW and glpF fused with crtZ in pSC102 | This study |
| pCrtW-CrtZ | crtW fused with crtZ in pSC102 | This study |
| pGlpF-CrtW-CrtZ | glpF fused with crtW and crtZ in pSC102 | This study |
| pPhCCD1 | PhCCD1 in pACYC184M2-Pm46 | This study |
| pGlpF-PhCCD1 | glpF fused with PhCCD1 in pACYC184M2-Pm46 | This study |
| pMBP-PhCCD1 | MBP fused with PhCCD1 in pACYC184M2-Pm46 | This study |
| pSPompA-PhCCD1 | The signal peptide sequence of ompA fused with PhCCD1 in pACYC184M2-Pm46 | This study |