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. 2018 Oct 11;37:247. doi: 10.1186/s13046-018-0917-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — ARHGEF16 promotes migration and proliferation of glioma cells. a Detection of endogenous ARHGEF16 protein level in H4, U87, and U118 cells by western blotting. b Validation of glioma cell lines stably overexpressing ARHGEF16. ARHGEF16 protein level was compared between Flag-ARHGEF16 U87 or Flag-ARHGEF16 U118 cells and corresponding control cells. c, d Comparison of the migratory potential between ARHGEF16 U87 or ARHGEF16 U118 cells and their corresponding control cells with the transwell migration assay. n = 3, **, P < 0.01. e-h Comparison of the proliferative capacity of ARHGEF16 U87 or ARHGEF16 U118 cells and their corresponding control cells with EdU assay (e, f) and soft agar colony formation assay (g, h). n = 3, **, P < 0.01