Fig. 4.

ARHGEF16 knockdown suppresses glioma growth. a, b Validation of ARHGEF16 knockdown in H4 cells; ARHGEF16 mRNA (a) and protein (b) levels were compared between sh-Control cells and sh-ARHGEF16 H4 cells. n = 3, **, P < 0.01. c-f Comparison of the proliferative capacity between sh-ARHGEF16 and control H4 cells with plate clone formation assay (c, d) and EdU assay (e, f). n = 3, **, P < 0.01. g, h ARHGEF16 mRNA (g) and protein (h) levels were detected to validate ARHGEF16 knockdown in U118 cells. n = 3, **, P < 0.01. i Growth curves of the U118 sh-Control and U118 sh-ARHGEF16 xenografts in nude mice. n = 5, **, P < 0.01. j Harvested glioma xenografts formed by the indicated U118 glioma cell lines. k Weight of the indicated glioma xenograft tumors. n = 5, **, P < 0.01. l The decreased ARHGEF16 protein level in U118 sh-ARHGEF16 xenografts compared to U118 sh-Control xenografts via western blotting