Fig. 1.

Screening of candidate long non-coding RNAs (lncRNAs) in the porcine granulosa cell transcriptome and classification of novel lncRNAs. a Schematic diagram of the pipeline used for the identification of lncRNAs in porcine granulosa cells. b Venn diagram presenting the results of the coding potential analysis of the obtained 4,160 long transcripts. Please note that five different tools (CPC, CNCI, PLEK, Pfam, FEELnc) were employed to analyze the coding potential. In consequence, 1,689 potentially non-coding long transcripts were identified and designated as candidate lncRNAs. c Classification of the obtained novel lncRNAs according to their genomic positions. *Customized algorithms of the authors were applied for the lncRNA identification in this step