Fig. 4.

Heatmap illustrating the expression profile of differentially expressed lncRNAs (presented as Z-score values) in porcine granulosa cells treated with TCDD for 3, 12 or 24 h. Red blocks represent up- and green blocks represent down-regulated lncRNAs. The color scale of the heatmap shows the expression level where the brightest green stands for − 2.0 Z-score and the brightest red stands for + 2.0 Z-score. Z-score was calculated using logFPKM and the following equation: [x - mean(x)] / sd(x) where ‘sd’ means standard deviation. C 3–24: untreated porcine granulosa cells (control) cultured for 3, 12 or 24 h. TCDD 3–24: porcine granulosa cells treated with TCDD (100 nmol/L) for 3, 12 or 24 h. *transcript expression was not detected in control (untreated) cells; ○ transcripts with expression level altered in two or three examined incubation times