Fig. 2.

Heat shock with busulfan treatment induced testis weight reduction and germ cell loss. (a) Testicular weight was significantly lower in the heat shock+busulfan(t) group than in the control and heat shock groups 28 days after treatment, but there was no significant difference between the heat shock+busulfan(t) group and busulfan i.p. group. (b) The number of germ cells in the heat shock+busulfan(t) group substantially decreased, similar to that of busulfan-treated mice. (c) The percentage of empty tubules. Bar = 50 μm.

a, b Different letters indicate a statistically significant difference (p < 0.05).