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. 2018 Sep 6;27(10):1451–1458. doi: 10.1177/0963689718794126

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 4. — Donor SSCs formed colonies and donor-derived spermatogenesis in the testes of mice in heat shock+busulfan(t) group. (a) Donor testis with ubiquitous expression of EGFP. Bar = 1 mm. (b) Recipient testis before SSC transplantation. Bar = 1 mm. (c) There are many donor-derived EGFP-positive colonies within recipient testis 2 months after transplantation. Bar = 1 mm. (d) Donor-derived SSCs formed spermatogenesis in the testes of recipients in the heat shock+busulfan(t) group 2 months after germ cell transplantation. ▴ Donor germ cell-derived spermatogenesis in recipient testis showed by GFP immuohistochemical staining with GPF antibody. Bar = 40 μm.