Fig. 4.

hAEC treatment inhibits macrophage and T-cell infiltration of retina. EAU rats were treated with hAECs or vehicle solution (BSS) by subretinal injection on day 0 and day 6 after immunization and sacrificed for tissue harvest on day 12 and day 18 after immunization (the peak and stable stage of the disease, respectively). The retinal infiltration of macrophages and T-cells was determined by immunofluorescence staining of CD68 and CD3, respectively. Representative immunofluorescence images in retinas harvested on day 12 and day 18 are shown in (A) and (D), respectively, with quantification of infiltrated immune cells in (B, C) and (E, F), respectively.

Data are presented as the mean ± SEM. n = 6 in each group. *p < 0.05; **p < 0.01; ***p < 0.001. Statistical analysis was performed using an unpaired Student’s t test. Scale bar=100 µm.

BSS: balanced salt solution; EAU: experimental autoimmune uveitis; hAEC: human amniotic epithelial cells; SEM: standard error of the mean.