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. 2018 Sep 11;27(10):1535–1547. doi: 10.1177/0963689718797570

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 3. — The differentiation and proliferation of EPCs and fibroblasts in plasma gel in co-culture medium containing 2% or 20% FBS. (A) Immunofluorescent staining for the pre-vascularization of EPCs using a labeled CD31 marker in plasma gel with the different co-culture media at 1, 3, 5 and 7 days. (B) The proliferation of EPCs in co-culture medium was observed at 1, 3, 5 and 7 days. (C) The proliferation of fibroblasts in co-culture medium was investigated at 1, 3, 5 and 7 days. *: indicates P < 0.05; **: indicates P < 0.01; ***: indicates P < 0.001 when compared with the co-culture medium containing 2% FBS.