Figure 8.

Cardiac structural and morphological changes in MI mice induced by high-dose metformin. (A) The expression levels of LC3b-1 and LC3b-2 were detected in the myocardium after treatment with different doses of metformin using Western blot analysis. (B) After high-dose metformin (200 mg/kg/d) treatment for 3 days, cardiomyocyte apoptosis was detected in the mouse hearts using TUNEL staining. (C) After high-dose metformin (200 mg/kg/d) treatment for 3 days, myocardial fibrosis was detected in the MI mice using Masson staining.