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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Jul 22;1863(10):1164–1178. doi: 10.1016/j.bbalip.2018.07.004

Figure 5.

Figure 5.

Sterol C24-methylation activity in Acanthamoeba. (A) Proposed sterol C24-methylation pathway in Acanthamoeba showing incorporation of SAM and [methyl-2H3]SAM = D3-SAM at C24 and C28 of the sterol side chain. (B) Mass spectral analysis of sterols (ergosterol, erg, poriferasterol, por, and 7-dhydroporiferasterol, DHP) recovered from trophozoites incubated in the presence and absence of [methyl-2H3]methionine.