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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Jul 22;1863(10):1164–1178. doi: 10.1016/j.bbalip.2018.07.004

Figure 7.

Figure 7.

Effects of ergosterol biosynthesis inhibitors as single or combination treatment on Acanthamoeba growth and encystment prepared by Method-1 (Materials and Methods). Panel A, structures of growth inhibitors. Panel B, Time-kill studies of 25-azacycloartanol (AZC) and voriconazole (VOR). Panel C, Quantification of AZC treated cysts (viable and clumped non-viable) and dead cells determined microscopically and by trypan blue staining. Panel D. A. castellanii treated with VOR and AZC at the previously determined IC50 concentration and then using the pair of compounds at varied concentration of the inhibitor from 16 nM to 3 μM. Data represent mean ±sd. P < 0.05, determined by T-test. In each case data are from a single experiment which is representative of three independent experiments.