Table 1.

Dilution and concentration of M17 medium. Amount of nitrogen (soymeal (5g/L), peptone from meat (2.5g/L), peptone from casein (2.5g/L), yeast extract (2.5g/L), and meat extract (5g/L)) in M17:17.5 g/L. Amount of carbon in M17:D-(+)-lactose:5.0 g/L.

Concentration of M17	Amount of Nitrogen (g/L)	Amount of Carbon (g/L)
(75% diluted: 9.3 g/L)	4.38	1.25
(50% diluted: 18.6 g/L)	8.75	2.50
(25% diluted: 27.9g/L)	13.13	3.75
M17 (control) (equivalent to 100%)	17.50	5.00
Addition of 25% extra m17 (37+9.3 g/L) of media (equivalent to 125%)	21.88	6.25
Addition of 50% extra M17 (37 + 18.6 g/L) of media (equivalent to 150%)	26.25	7.50