Table 3.
Effect of different concentrations of M17 medium on growth of P. acidilactici kp10 and BLIS production. The results were recorded after 24 h of fermentation.
| Concentration of M17 (%) | Cell Growth ( O D 600nm ) | Dry Cell Weight (g/L) | pH | BLIS Activity (AU/mL) | Yield (AU/g cell) | Productivity (AU/g cell/h) |
|---|---|---|---|---|---|---|
| 25 | 0.78 (±0.07)d |
2.9 (±0.03)c |
6.88 (±0.01)a |
1189.67 (±163.08)d |
4.1 x 102 (±0.18)d |
17.08 (±0.67)d |
|
| ||||||
| 50 | 1.17 (±0.02)a |
4.4 (±0.01)a |
6.70 (±0.08)a |
2855.33 (±185.45)a |
6.5 x 102 (±0.13)b |
27.08 (±0.07)b |
|
| ||||||
| 75 | 1.17 (±0.05)a |
4.3 (±0.02)a |
6.43 (±0.07)b |
2855.33 (±175.63)a |
6.6 x 102 (±0.07)b |
27.50 (±0.09)b |
|
| ||||||
| 100 | 1.19 (±0.00)a |
4.4 (±0.00)a |
6.45 (±0.06)b |
2855.33 (±181.71)a |
6.5 x 102 (±0.17)b |
27.08 (±0.05)b |
|
| ||||||
| 125 | 1.04 (±0.02)b |
3.6 (±0.02)b |
6.53 (±0.01)b |
1843.07 (±36.71)c |
5.1 x 102 (±0.15)c |
21.25 (±0.14)c |
|
| ||||||
| 150 | 0.95 (±0.00)c |
3.5 (±0.00)b |
6.50 (±0.02)b |
2467.66 (±94.71)b |
7.1 x 102 (±0.21)a |
29.58 (±0.74)a |
The results presented are the average of triplicate experiments and are expressed as mean ± standard deviation. a,b,c,dMeans values in the same column expressed with different superscript letters are significantly different at P< 0.05.