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. 2018 Aug 11;24:5588–5597. doi: 10.12659/MSM.906857

Figure 2.

Figure 2

Silencing of TLR4/COX-2 inhibits PCa cell proliferation. PCa3 cells were transfected with PBS (control), TLR4 siRNAs (si-TLR4), COX-2 siRNAs (si-COX-2), COX-2 siRNAs plus TLR4 siRNAs (si-COX-2+si-TLR4), negative control (NC). (A) The protein expression levels of TLR4 and COX-2 were measured by western blot assays, and the results were analyzed according to the protein grayscales (*** P<0.001). (B) The mRNA expression levels of TLR4 and COX-2 were detected by qRT-PCR assays (* P<0.05, ** P<0.01, *** P<0.001). (C) The proliferation ability was analyzed by CCK-8 assays (** P<0.01, *** P<0.001 vs. NC group; # P<0.05, ## P<0.01, ### P<0.001 vs. si-COX-2+ si-TLR4 group). (D) The proliferation ability was also detected by CFSE assays (*** P<0.001). TLR4 – toll-like receptor 4; COX-2 – cyclooxygenase-2; PCa – prostate cancer.