Skip to main content
. 2018 Aug 11;24:5588–5597. doi: 10.12659/MSM.906857

Figure 4.

Figure 4

Silencing of TLR4/COX-2 decreases MMP-2, MMP-9, and E-cadherin expressions, and increases TIMP-1 and vimentin expressions. (A) qRT-PCR assays were performed to analyze the expression levels of MMP-2, MMP-9, TIMP-1, E-cadherin, and vimentin in treated PCa3 cells, GAPDH was used as an internal control, (* P<0.05, ** P<0.01, *** P<0.001). (B) Western blot assays were used to detect MMP-2, MMP-9, TIMP-1, E-cadherin, and vimentin expressions in treated PCa3 cells, β-actin was used as an internal control, (* P<0.05, ** P<0.01, *** P<0.001). TLR4 – toll-like receptor 4; COX-2– cyclooxygenase-2; PCa – prostate cancer; MMP – matrix metalloproteinase; TIMP – tissue inhibitor of matrix metalloproteinases; E-cadherin – epithelial-cadherin.