Figure 4.

PUX10 Can Recruit At-CDC48A to LDs in Pollen Tubes with the Help of Its UBX Domain.

The proteins were transiently expressed in tobacco pollen tubes as N-terminal fusions to the fluorescent protein mVenus or mCherry as indicated. The tubes were cultivated for 5 to 8 h, fixed with formaldehyde, and stained with the blue fluorescent LD dye monodansylpentane (MDH; [B], [C], and [E]) or Nile Red (D). Then, they were monitored by confocal microscopy. At-CDC48A expressed alone is localized in the cytosol (A) but is recruited to LDs by Nt-PUX10c (B). Nt-PUX10c missing the C-terminal UBX domain (δ379-465) still localizes to LDs but can no longer recruit At-CDC48A (C). At-PUX10 alone localizes to LDs (D) and can also recruit At-CDC48A. Images are representative of 11 (A), 9 (B), 10 (C), 12 (D), and10 (E) pollen tubes. Bars = 10 µm.