Figure 7.

LD Proteins Are Enriched in the pux10 Mutant Seedlings.

(A) In the qrt pux10-1 line, LD proteins are significantly more abundant at 1 and 2 DAI when compared with the qrt PUX10 line. This effect is partially rescued in the complemented lines. Proteins were isolated from total seedling homogenate of rehydrated seeds (0 DAI) and seedlings at 1, 2, and 3 DAI. The same experiment was independently conducted on seeds and seedlings of Ws-4 and pux10-3. Here, the observed difference is greatest at 0 and 1 DAI. LC-MS/MS data were processed with MaxQuant software’s LFQ algorithm. Values are given as ‰ of total LFQ intensities. Error bars correspond to sd, when comparing the summed up intensities of the 13 given proteins between the three biological replicates. The same summed up intensities for each biological replicate were used for one-factor ANOVA followed by Tukey’s post-hoc test; lowercase letters indicate statistical significance, P < 0.05.

(B) The same values as in (A) were normalized for each protein to the highest value in all compared lines and time points. Green shows high abundance and dark red not detectable. For separate graphs, see Supplemental Figure 18, and for all individual values, see Supplemental Data Sets 18 and 29.