Figure 3.

Knocking out PGI1 Decreases Levels of Bioactive GAs and Increases Levels of Inactive GAs.

Contents of the indicated GAs (pg mg⁻¹ DW) in shoots (20 d after sowing) of Ws-2 and pgi1-2 plants, and pgi1-2 plants transformed with promPGI1:PGI1(1). Plants were harvested at the end of the light phase. Active GAs (significantly less abundant in pgi1-2 plants than in Ws-2 plants) and inactive GAs (significantly more abundant in pgi1-2 plants than in Ws-2 plants) are highlighted in orange and green, respectively. Values represent the means ± se of two independent experiments, each consisting of four biological replicates corresponding to a pool of shoots from four plants. Asterisks indicate significant differences with respect to Ws-2 according to ANOVA (*P < 0.05, **P < 0.01, and ***P < 0.001).