The Low FA Content Phenotype of pgi1-2 Seeds Is Not Due to Reduced Active CK or GA Contents.
(A) to (C) External phenotype (A), specific weight (B), and total FAME contents (C) of wild-type (Col-0), 35S:AtCKX1, and ga20ox1 ga20ox2 dry seeds.
(D) and (E) Specific weight (D) and total FAME contents (E) of dry seeds of Ws-2 and pgi1-2 plants following exogenous CK and GA4+7 application.
Bar in (A) = 200 μm. Values in (B) to (E), represent the means ± se of four biological replicates obtained from three independent experiments, each biological replicate being a pool of 300 seeds ([B] and [D]) or 20 seeds ([C] and [E]). In (B) and (C), asterisks indicate significant differences from wild-type seeds according to Student’s t tests (P < 0.05).