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. 2018 Aug 22;30(9):1954–1970. doi: 10.1105/tpc.18.00287

Figure 1.

Figure 1.

Multiple Endogenous Loci Show DNA Hypermethylation upon APE2 Depletion or ZDP/APE2 Double Depletion.

(A) Analysis of DNA methylation levels at loci DT-75, DT-76, DT-77, and DT-78 by chop-PCR. AvaI, HpaII, and BstUI are DNA methylation-sensitive restriction enzymes. DNA hypermethylation results in increased levels of the PCR products. Undigested DNA was amplified as a control. DT-75, DT-76, DT-77, and DT-78 reside in 3′ regions of genes AT1G26380, AT1G26390, AT1G26400, and AT1G26410, respectively. Also see Supplemental Figure 2B for the precise genomic locations of these DT loci.

(B) Analysis of DNA methylation levels at loci DT-75, DT-77, and DT-78 by locus-specific bisulfite sequencing. The overall percent methylation of cytosine sites in different sequence contexts is presented.