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. 2018 Aug 10;30(9):2197–2213. doi: 10.1105/tpc.18.00293

Figure 1.

Figure 1.

ANAC087 and ANAC046 Are Expressed in the Root Cap.

(A) A schematic representation of the Arabidopsis root tip. The columella is shown in yellow, the LRC in green, the epidermis in pink, and the quiescent center in red. The different developmental stages of the root cap are indicated. (Adapted from Kumpf and Nowack, 2015.)

(B) The pANAC087:ANAC087-VENUS reporter was expressed in the entire root cap and showed weaker expression in some epidermal cells and in the differentiating TE cells (elongated nucleus indicated by arrowhead in the inset). Shown is the root of a representative 5-d-old T3 seedling. Five homozygous lines were analyzed in T3.

(C) The pANAC046:ANAC046-VENUS reporter was expressed in the columella and showed expression in some epidermal cells and in the differentiating TE cells (elongated nucleus indicated by arrowhead in the inset). Shown is the root of a representative 5-d-old T3 seedling; two homozygous lines were analyzed in T3. In (B) and (C), the VENUS signal is shown in yellow, and roots are shown without (left) and with (right) transmitted light. The VENUS signal is shown as a Z-projection, whereas the bright-field channel is shown as a single stack. Bars in (B) and (C) = 50 µm.