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. 2018 Aug 10;30(9):2197–2213. doi: 10.1105/tpc.18.00293

Figure 3.

Figure 3.

Expression of BFN1, EXI1, and RNS3 Is Regulated by ANAC087, ANAC046, and SMB.

(A) RT-qPCR analysis showed reduced expression of PCD-associated genes (BFN1, EXI1, and RNS3) in root tips of the anac087-1 mutant (root tips were cut just above the LRC), whereas these genes were upregulated in the ANAC087-inducible overexpression line. t test; *P < 0.05, **P < 0.01, and ****P < 0.0001.

(B) RT-qPCR analysis for small root tips of the anac046-1 mutant (root tips were cut just above the columella) and the ANAC046-inducible overexpression line. Expression of ANAC046 is not altered in the anac087-1 mutant or ANAC087 overexpression line and vice versa. The error bars in (A) and (B) show se of three independent biological replicates (three independent treatments and three technical repeats each). t test; *P < 0.05 and **P < 0.01.

(C) PCD-associated genes showed increased expression upon induction of the p35S:SMB-GR (shown as SMB-GR) and decreased expression in the smb mutant (root tips were cut just above the LRC), as indicated by RT-qPCR analysis. As application of dexamethasone (dex) only leads to activation of the highly expressed SMB-GR fusion protein, SMB RNA expression is not increased. The error bars show se of three independent biological replicates (three independent treatments and three technical repeats each). t test; *P < 0.05, ***P < 0.001, and ****P < 0.0001.