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Altered distribution of cell wall materials and F-actin cytoskeleton in RNAi pollen tubes. Wild-type (WT) and RNAi pollen tubes cultured 5 h in vitro were collected and stained with Ruthenium Red (A; esterified pectins), Toluidine Blue O (B; deesterified pectins), and Alexa Fluor 488 phalloidin (E; actins) and labeled with LM20 (C; esterified pectins) and LM19 (D; deesterified pectins). Bars = 10 μm.
