Figure 5.

Red light-induced stomatal opening in whole leaves from PM H⁺-ATPase mutants. Mature leaves from aha1-9 (A) or aha2-5 (B) and Col-0 were harvested from dark-adapted plants. The harvested leaves were kept in the dark (Dk) for 60 min or illuminated with red light (RL; 600 µmol m^–2 s^–1) for 15, 30, 45, or 60 min. Data represent means of representative values from three independent measurements with sd. A, Asterisks denote statistically significant decrements of the mean of aha1-9 compared with that of Col-0 at each time point. N.S., Not significant (one-tailed Student’s t test: *, P < 0.05; **, P < 0.001; and N.S., P > 0.07). B, The mean of aha2-5 was not statistically significantly lower than that of Col-0 at each time point (one-tailed Student’s t test: N.S., P > 0.6).