Table 1. Summary of the (Z)-3-hexenal reductase purification process from Arabidopsis leaves.
Purification Step | Total Protein | Total Activity | Specific Activity | Purification | Yield |
---|---|---|---|---|---|
mg | nkat | nkat mg−1 | -fold | % | |
Crude extract | 1,707 | 1,062 | 1.66 | 1 | 100 |
Ammonium sulfate | 1,634 | 592 | 26.0 | 0.58 | 55.7 |
Butyl-Toyopearl | 83.7 | 90.0 | 10.8 | 1.73 | 8.47 |
Hydroxyapatite | 0.28 | 21.2 | 6.62 | 119 | 1.99 |
HiTrap DEAE | 0.031 | 3.94 | 7.88 | 209 | 0.37 |