GSH turnover in F. robusta and F. australasica. GSH concentrations in shoots (A) and roots (B) of 20-d-old seedlings of F. robusta (C3) and F. australasica (C4) in a time course of 48 h after transfer to medium supplemented with 2 mm BSO. Data are presented as means and se, n = 4. C, GSH synthesis was analyzed in 20-d-old seedlings exposed to low sulfate (20 µm sulfate; S20) or adequate sulfate (750 µm sulfate; S750) for 6 d by resupply with 0.2 mm [35S]sulfate solution for 4 h. GSH consumption rate is calculated from A at 4 h after treatment with 2 mm BSO. Data are shown as box plot (25%–75%); the line represents median, and the whiskers represent 1.5 IQR, n = 4. D Transcript levels of GGCT2.1 in shoots and roots of 20-d-old seedlings in a time course of 48 h after transfer to medium supplemented with 2 mm BSO. Data are presented as means and SEM, n = 4. Asterisks represent significant differences between F. robusta and F. australasica at P < 0.05 (Student’s t test).