Figure 1.
Identification of an EAR-motif-containing transcription factor IDS1 in rice using a salt tolerance assay. A, Pie chart showing different types of EAR-motif-containing TFs with increased or decreased salt tolerance in their amiRNA interference seedlings. B, Salt tolerance phenotype of IDS1-knockdown rice transgenic plants. The seedlings of wild-type (WT) and amiRNA-mediated IDS1-knockdown lines RI-1, RI-2, and RI-3 were separately treated with or without (control) 120 mm NaCl for 10 d (n ≥ 30). C, The statistical quantification of survival rates of wild-type NIP and the three independent IDS1 knockdown transgenic lines (n ≥ 30). D and E, Determination of the Pro and MDA contents in wild-type NIP and IDS1-knockdown transgenic lines with or without NaCl treatment. Error bars represent sds (n ≥ 30). F, Salt tolerance phenotypes of IDS1 overexpression (IDS1-OE), and ids1-1 mutant rice plants. G, Survival rates of wild-type NIP, IDS1-OE, and ids1-1 mutant seedlings as well as wild-type DJ seedlings treated with or without (control) 120 mm NaCl for 14 d (n ≥ 30). Error bars represent sds among three independent replicates. **P < 0.01 (Student’s t test). Scale bar, 5 cm.
