Figure 2.

IDS1 confers transcriptional repression activity. A, Subcellular localization of IDS1. Arrows mark the nuclei. NLS, nuclear localization sequence. Scale bars, 10 μm. B, Wild-type and loss-of-function mutated amino acid sequence of IDS1 EAR motif. C and D, Transient dual-luciferase expression assays illustrating the transcriptional repression activity of IDS1. The reporters luciferase (LUC) and renilla luciferase (REN) and the effectors (pBD, pBD-IDS1, pBD-VP16, and pBD-IDS1-VP16) were separately constructed as shown in the left columns of C and D. The activities of LUC and REN were determined 16 h after transformation, and the relative LUC/REN ratios represent the transcriptional activation activities. Error bars represent sds among three independent replicates. **P < 0.01 (Student’s t test).