Skip to main content
. 2018 Aug 27;178(2):876–889. doi: 10.1104/pp.18.00737

Figure 5.

Figure 5.

GhCPK33-phosphorylated GhOPR3 shows decreased protein stability. A, Diagrams of the modified dual-luciferase reporter system. 35S, Promoter of cauliflower mosaic virus 35S RNA gene; REN, Renilla luciferase; CaMV term, cauliflower mosaic virus terminator; LUC, firefly luciferase. B, LUC activity indicates the accumulation level of GhOPR3 with or without GhCPK33 in N. benthamiana leaves. C, GhOPR3-LUC protein levels in cotton protoplasts following treatment with empty vector (EV) or GhCPK33. The LUC/REN activity ratios are means ± sd, n = 3. D, LUC activity indicates the level of GhOPR3, GhOPR3-mA, or GhOPR3-mD in the presence of GhCPK33 in N. benthamiana leaves. E, GhOPR3-LUC, GhOPR3-mA-LUC, or GhOPR3-mD-LUC protein accumulation levels of the reporter in the presence of GhCPK33 in cotton protoplasts. The LUC/REN activity ratios are means ± sd, n = 3. F, Western blotting showing the accumulation of GhOPR3-MYC, GhOPR3-mA-MYC, or GhOPR3-mD-MYC when GhCPK33 also is expressed. All the experiments were repeated at least three times with similar results. Bars in B and D = 1 cm.