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. 2018 Aug 27;178(2):783–794. doi: 10.1104/pp.18.00725

Figure 7.

Figure 7.

Long-distance movement of tomato PEBP gene mRNA in tobacco-tomato heterografting experiments. A, Representative image of tobacco-tomato heterografting experiments, depicting a wild-type tobacco scion grafted with a wild-type tomato stock. The grafting union was secured by Parafilm (white arrow). Mature leaves of the tobacco scions were removed to enhance the sink strength. B, RT-PCR analysis of mRNA from wild-type tomato (Tom), wild-type tobacco (Tob), or tobacco scions (indicated by stars) grafted onto tomato stocks (Tob*/Tom). PCR was performed with gene-specific primers for tomato SP2G (MFT-like clade); SP3D, SP5G, and SP11A (FT-like clade); SP9D (TFL-like clade); and loading controls of tomato, IMPORTINα (SIKAPα; first lane 1), or tobacco, ACTIN2 (NtACT2; lanes 2 and 3).