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Proliferation of hESC-derived CEC. (A) Growth curve of hESC and hESC-derived CEC monitored on IncuCyte for 66 hours (n = 3). (B) Flow cytometric cell cycle analysis of PI-stained hESC and hESC-derived CEC. (C) Immunostaining for KRT15 and Ki67 on CEC. Scale bar: 50 μm. (D) Quantification of Ki67+/KRT15+ cells among CEC derived from two hESC lines H9 and CT3 (n = 5).
