Table 2.

Comparison of the features of Micro-CT, LAT, and Paraffin sectioning used in the visualization of the internal structure of bark and ambrosia beetles

Methods	Time to sample beetle	Sample preparation	Resolution	Invasive to specimens	Price ($)	References
LAT (LATscan)	2–5 h	Dry	Depend on microscope and camera	Yes	1,100 (in 2016)	http://plantscience.psu.edu/research/labs/roots/sites/laserfacility
Micro-CT	1–3 h	Dry	0.5–5 μm	No	100–200	Friedrich et al. 2014, Ruan et al. 2016
Paraffin section with brightfield microscopy	>24 h	Paraffin seal	Depend on microscope and camera, but >3 μm	Yes	50–200	Bancroft and Gamble 2008, Gijtenbeek et al. 2006, Lowe and Anderson 2014

Time and price are estimated for one beetle specimen.