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. Author manuscript; available in PMC: 2019 Mar 17.
Published in final edited form as: Nat Med. 2018 Sep 17;24(10):1599–1610. doi: 10.1038/s41591-018-0158-8

Figure 3. YAP/TAZ are instrumental for BRD4 recruitment to chromatin.

Figure 3

a) ChIP-qPCR showing increased BRD4 binding on enhancers and promoters of YAP/TAZ targets upon YAP5SA overexpression in MCF10A cells, but not in the presence of JQ1 (1µM, 6h). ChIP with pre-immune IgG displayed background signal (which was comparable in all samples). DNA enrichment was calculated as fraction of input and is presented as fold vs. BRD4 binding in control cells.

b) RT-qPCR for representative YAP/TAZ target genes showing upregulation upon YAP5SA overexpression in MCF10A cells, but not in the presence of JQ1 (1µM, 24h) or upon depletion of BRD2/3/4 (siBRD mix A and B). Data are presented as individual data points (n=2 biologically independent samples) + average (bar), from one out of three experiments, all producing similar results. BET inhibition does not impair the expression of exogenous YAP (see Supplementary Fig. 3e).

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