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. 2018 Apr 24;16(11):1868–1877. doi: 10.1111/pbi.12922

Figure 2.

Figure 2

Analyses of T0 wild strawberry plants carrying CRISPR constructs revealed mutations in FveTAA1 and FveARF8 genes. (a) PCR genotyping results of T0 transgenic plants harbouring the JH19‐FveTAA1 construct. The presence of Cas9 PCR product is shown. (b) PCR genotyping results of T0 transgenic plants harbouring the JH19‐FveARF8 construct. The presence of Cas9 (top row) and sgRNA (lower row) in these transgenic plants is shown by the PCR bands. (c) Sequencing of PCR products revealed the presence of mutations in the FveTAA1 gene, more abundantly around the PAM1 (TGG) site. Individual transgenic plants are named with a number shown to the left. 11/23/26 refer to three different plants having the same genotype. All mutations exist as heterozygous. (d) Sequencing of PCR products revealed the presence of mutations in the FveARF8 gene at the PAM2 (TGG) site only. Individual plant names (numbers) are to the left of the sequence. Mutations exist as heterozygous, homozygous or biallelic.