Table 1.
Summary of genotyping results in T0 transgenic lines
| Gene name and plant no. | Line no. | Genotypea | PAM site | Confirmation method | Notes |
|---|---|---|---|---|---|
| JH19‐TAA1‐1 | Line 5 | (0, −1a) | PAM1(340 bp) | Sequencing | |
| JH19‐TAA1‐3 | Line 207 | NA | PAM2(470 bp) | Sequencing | |
| JH19‐TAA1‐7 | Line 208 | NA | PAM2(470 bp) | Sequencing | |
| JH19‐TAA1‐8 | Line 208 | NA | PAM1(340 bp) | Sequencing | Mismatch starts 240 bp upstream of PAM1 |
| JH19‐TAA1‐9 | Line 208 | NA | PAM2(470 bp) | Sequencing | |
| JH19‐TAA1‐11 | Line 5 | (0, −1a) | PAM1(340 bp) | Sequencing | Figure 2 |
| JH19‐TAA1‐13 | Line 17 | (0, 1a) | PAM2(470 bp) | Sequencing | Figure 2 |
| JH19‐TAA1‐14 | Line 209 | NA | PAM2(470 bp) | Sequencing | |
| JH19‐TAA1‐20 | Line 35 | (0, −1a+1S) | PAM1(340 bp) | Sequencing | Figure 2 |
| JH19‐TAA1‐22 | Line 8 | NA | PAM1(340 bp) | Sequencing | |
| JH19‐TAA1‐23 | Line 39 | (0, −1a) | PAM1(340 bp) | Sequencing | |
| JH19‐TAA1‐25 | Line 109 | NA | PAM2(470 bp) | Sequencing | |
| JH19‐TAA1‐26 | Line 7 | (0, −1a) | PAM1(340 bp) | Sequencing | Figure 2 |
| JH19‐TAA1‐45 | Line 200 | NA | PAM2(470 bp) | Sequencing | |
| JH19‐TAA1‐54 | Line 5 | NA | PAM1(340 bp) | Sequencing | |
| JH19‐TAA1‐60 | Line 6 | NA | PAM1(340 bp) | Sequencing | |
| JH19‐TAA1‐306 | Line 8 | (0, −1b) | PAM1(340 bp) | Sequencing | Figure 2 |
| JH19‐ARF8‐4 | Line 102 | (−2, −3) | PAM2(360 bp) | Sequencing | Figure 2 |
| JH19‐ARF8‐5 | Line 102 | (0, −2) | PAM2(360 bp) | Sequencing | Figure 2 |
| JH19‐ARF8‐66 | Line 15 | (0, −4) | PAM2(360 bp) | Sequencing | Figure 2 |
| JH19‐ARF8‐67 | Line 15 | (−2, −2) | PAM2(360 bp) | Sequencing | |
| JH19‐ARF8‐72 | Line 1 | (0, −2) | PAM2(360 bp) | Sequencing | |
| JH19‐ARF8‐100 | Line 103 | (−2, −2) | PAM2(360 bp) | Sequencing | Figure 2 |
| JH19‐ARF8‐146 | Line 19 | (−2, −11 + 1S) | PAM2(360 bp) | Sequencing | Figure 2 |
| JH19‐ARF8‐147 | Line 19 | (−2, −2) | PAM2(360 bp) | Sequencing | Figure 2 |
| JH19‐ARF8‐149 | Line 13 | NA | PAM2(360 bp) | Sequencing | Large fragment deletion |
| JH19‐ARF8‐154 | Line 1 | NA | PAM1(340 bp) | Sequencing | Mismatch starts 140 bp upstream of PAM1 |
| JH19‐ARF8‐105 | Line 13 | NA | PAM1(340 bp) | Sequencing | Mismatch starts 150 bp upstream of PAM1 |
| JH19‐ARF8‐156 | Line 7 | (−2, −2) | PAM2(360 bp) | Sequencing | |
| JH19‐ARF8‐157 | Line 7 | (−2, −2) | PAM2(360 bp) | Sequencing | |
| JH19‐ARF8‐116 | Line 29 | NA | PAM2(360 bp) | Sequencing | |
| JH19‐ARF8‐391 | Line 23 | (0, −1 + 1S) | PAM2(360 bp) | Sequencing | 1 bp deletion and 1 bp substitution |
a
− sign indicates deletion, 0 indicates no change. −1a, −1b refer to different 1 bp deletion alleles. 1S stands for single base substitution. NA: sequencing results are too complex to resolve, usually due to genetic mosaics and multiple alleles.