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. 2018 Apr 30;16(11):1918–1927. doi: 10.1111/pbi.12927

Figure 1.

Figure 1

Gene editing of rice eIF4G. (a) Schematic illustration of the eIF4G locus (LOC_Os07g36940) composed of 12 exons (black boxes) and 11 introns (black lines); the YVV residues known to be associated with reactions to RTSV are indicated with a grey box and the gRNA target sequences are represented as red triangles. (b) Schematic diagrams of CRISPR/Cas9 vectors. TaU6, Triticum aestivum U6 promoter; ZmUBI1, Zea mays ubiquitin promoter; Cas9, CRISPR‐associated protein‐9 nuclease gene; PolyA, termination signal; CaMV35S, Cauliflower mosaic virus 35S promoter; Hygromycin, hygromycin resistance gene.