Table 1.
Vector ID | Vector description | No. of immature embryos | No. of regenerated plants | No. of transgenic events | Transformation efficiency (%) | No. of events with mutations | Mutation rate (%) |
---|---|---|---|---|---|---|---|
1146 | pCas9‐eIF4G‐gRNA1 | 50 | 25 | 25 | 50.0 | 9 | 36.0 |
1147 | pCas9‐eIF4G‐gRNA2 | 50 | 15 | 15 | 30.0 | 13 | 86.6 |
1148 | pCas9‐eIF4G‐gRNA3 | 50 | 32 | 32 | 64.0 | 21 | 65.6 |
Total | 150 | 72 | 72 | 48.0 | 43 | 59.7 |