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. 2018 Apr 17;16(11):1878–1891. doi: 10.1111/pbi.12923

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© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The interactions between OsPK2 and other PKps (a) yeast two‐hybrid assays showed interactions between OsPK2 (PKpα1, α1) and three putative pyruvate kinase plastidic isozymes, PKpα2 (α2), PKpβ1 (β1) and PKpβ2 (β2). Serial dilutions (10‐fold) of yeast cells expressing the indicated proteins were plated onto nonselective medium (SD/‐Leu/‐Trp) (left) or selective medium (SD/‐Leu/‐Trp/‐Ade/‐His) (right). The interactions between pGADT7‐T (T) and pGBKT7‐53 (53), pGADT7 (AD) and pGBKT7 (BD) were used as the positive and negative controls, respectively. (b) BiFC assay showed that OsPK2 (PKpα1, α1) can interact with PKpα2 (α2), PKpβ1 (β1) and PKpβ2 (β2), and PKpβ1 (β1) also can interact with PKpβ2 (β2) in the chloroplast of tobacco cells. Bars = 30 μm.