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. 2018 May 15;16(11):1954–1967. doi: 10.1111/pbi.12930

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© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Snapshot displaying SNP genotyping with KASP assays using markers flanking the major QTLs for ELS and LLS. Different scenarios of validation of major QTL flanking SNPs B05_22527171 (a‐c) and A06_14301316 (d‐f) associated with ELS, A05_20406182 (g‐i), A03_134198144 (j‐l) and A05_82270000 (m‐o) associated with LLS segregating in the RIL population. Marker genotyping data generated for each genotype were viewed using the SNPviewer software (LGC Genomics). The scatter plot along x and y axes represents allelic discrimination for a particular marker in the examined population. Red and blue clusters represent the homozygous alleles showing polymorphism.