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. 2018 Jul 3;72(4):167–175. doi: 10.1097/FJC.0000000000000612

FIGURE 1.

FIGURE 1.

CTRP9 subdues the formation of foam cell induced by ox-LDL in THP-1 macrophages. A, Impact of different CTRP9 concentrations on viability of foam cell derived from THP-1 macrophage. THP-1 macrophages undertook a 24-hour culture with ox-LDL (50 µg/mL), followed by the 24-hour addition of increasing doses of CTRP9 (0–10 μg/mL). The CCK-8 assay was adopted to detect the cell viability (mean ± SD). B, THP-1–derived macrophages accepted 24-hour culture in the presence or absence of ox-LDL (50 µg/mL). The media were then replaced, and macrophages were treated with or without CTRP9 with concentration of 0.3, 1, and 3 μg/mL, respectively, for another 24 hours. Then, Oil Red O as well as Harris hematoxylin were used to stain these macrophages. A microscope with magnification ×200 was used to observe the cells. C, Detect the cholesterol content to assess the lipid deposition. The methodology of measurement is given in material and methods. Values obtained represented the mean ± SD of 3 separated experiments. *P < 0.05 and ***P < 0.001 versus the ox-LDL group; ##P < 0.01 and ###P < 0.001 versus the control group.