Fig 7. Ethanolic extracts with TLR2 and TLR4 antagonistic activities in HEK-TLR2 and HEK-TLR4 cell lines.

HEK-TLR2 or HEK-TLR4 cells were incubated with extracts in different concentrations or vehicle (70% ethanol), followed by stimulation of HEK-TLR2 cells with Pam2CSK4 or HEK-TLR4 cells with LPS-EB Ultrapure. Viability was measured using the Alamar Blue Assay and was normalized to the negative control (untreated cells). TLR2 and TLR4 receptor activity were measured using SEAP production and were normalized to ethanol-treated cells. Data are displayed as receptor stimulation divided by normalized viability. The five extracts with the highest mitigation of LPS-induced inflammatory signal from Fig 4 are displayed here (for comparison of further extracts, see S4 Fig). Data represents means (n≥4).