Figure 6.
Increased LRP4 ubiquitination in aged mice. A, Increased Lrp4 mRNA levels in synaptic regions in aged mice; t(4) = 3.9, *p = 0.017 for Lrp4; t(4) = 6.04, *p = 0.019 for Musk, N = 3 mice per group, unpaired t test. B, Increased LRP4 protein level in MG132-treated C2C12 myotubes, but not chloroquine (CLQ)-treated myotubes. C, Quantification of data in B; F(2,6) = 50.4, ***p = 0.0002; N = 3, one-way ANOVA. D, Increased LRP4 ubiquitination in synaptic regions of aged muscles. Ubiquitinated proteins were isolated by anti-LRP4 antibody and probed with anti-Ubiquitin antibody. E, Quantification of data in D; t(4) = 4.6, *p = 0.031; N = 3 mice per group, unpaired t test.