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. 2018 Oct 10;38(41):8860–8873. doi: 10.1523/JNEUROSCI.0860-18.2018

Figure 9.

Figure 9.

Ameliorated NMJ fragmentation and denervation in aged mice by SGα overexpression. A, Reduced fragmentation and improved innervation. Shown were TA muscles of mice at different ages and indicated AAV treatment, whole-mount stained with CF568 α-BTX (red) and anti-NF/Syn antibodies (visualized by AlexaFluor 488 goat anti-rabbit IgG; green). Blue arrowhead, denervated or partially innervated endplate. Scale bar, 50 μm. B, Enlarged images of individual endplates. Scale bar, 10 μm. C–F, Quantitative analysis of data in A. C, Rescue of denervation in aged mice by SGα expression; F(2,12) = 29, ***p = 0.0001, **p = 0.0051. D, Rescue of fragmentation number in aged mice by SGα expression; F(2,12) = 25.8, ***p = 0.0001, **p = 0.0023. E, Rescue of fragmented endplate percentage in aged mice by SGα expression; F(2,12) = 93, ***p = 0.0001, ***p = 0.0003. F, Rescue of reduced AChR cluster intensity in aged mice by SGα expression; F(2,12) = 35.9, ***p = 0.0001, **p = 0.0028; N = 5 mice per group, one-way ANOVA.