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Our acute RD model evokes gliosis. Immunostaining of GFAP (a Müller gliosis marker, represented as green) and Ki67 (a proliferation marker, represented as red) was performed in RD retinae. Nuclei were stained by Hoechst (blue). The region of detachment from the RPE is represented as RD, in contrast to the normal region (represented as control). Scale bar, 100 μm. ONL, Outer nuclear layer; INL, inner nuclear layer.
