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RD-induced TRPV4 activation recruits macrophages in subretinal space. Immunostaining of CD11b (a macrophage marker, represented as green) was performed in RD retinae. Nuclei were stained by Hoechst (blue). Shown is quantification of CD11b-positive cell numbers in WT or TRPV4 retinae 24 h after retinal detachments (n = 8, **p = 0.00632, Student t test). Scale bar, 100 μm. ONL, Outer nuclear layer; INL, inner nuclear layer.
