Figure 4.

Müller glial TRPV4 is synergistically activated by mechanical stimuli and body temperature. A, Representative traces at 25 or 37°C induced by application of minimal positive pressure (+10 mmHg) by the HSPC system (red traces) with ramp pulses (−100 to +100 mV) compared with basal traces (black traces). The holding potential was at −60 mV. B, The outward rectified the current–voltage relationship of mechanical stimulus-evoked current (red trace) corresponding to the application of 10 mmHg pressure. The black trace represents the basal current–voltage relationship. C, Quantification of densities of positive pressure-evoked currents (n = 5, *p = 0.00056, Student t test).